LOCUS construct_1 5151 bp DNA linear 30-JAN-2022 FEATURES Location/Qualifiers misc_feature 1007..2021 /label="kanR" /color="#00ff00" CDS 1088..1882 /label="aph3'" /color="#ccffcc" gene 1088..1882 /label="aph3'" /color="#a6acb3" CDS 521..1006 /label="AmpR" /color="#ccffcc" misc_feature 5..520 /label="flank_A" /color="#a6acb3" CDS 493..520 /label="erm" /color="#993366" gene 493..520 /label="erm" /color="#a6acb3" promoter 4168..4272 /label="AmpR promoter" /color="#ffffff" CDS 4342..4647 /label="AmpR" /color="#ccffcc" misc_feature 2026..4167 /label="manP" /color="#ff0000" CDS 2189..4141 /label="manP" /color="#993366" gene 2189..4141 /label="manP" /color="#a6acb3" misc_feature 4648..5147 /label="flank_B" /color="#a6acb3" CDS 4648..5079 /label="erm" /color="#993366" gene 4648..5079 /label="erm" /color="#a6acb3" ORIGIN 1 GGAGtacgcg ttaacccggg cccgcggatg catatgatca gatcctttaa ctctggcaac 61 cctcaaaatt gaatgagaca tgctacacct ccggataata aatatatata aacgtatata 121 gatttcataa agtctaacac actagactta tttacttcgt aattaagtcg ttaaaccgtg 181 tgctctacga ccaaaactat aaaaccttta agaactttct ttttttacaa gaaaaaagaa 241 attagataaa tctctcatat cttttattca ataatcgcat ccgattgcag tataaattta 301 acgatcactc atcatgttca tatttatcag agctcgtgct ataattatac taattttata 361 aggaggaaaa aatatgggca tttttagtat ttttgtaatc agcacagttc attatcaacc 421 aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatat aaccaaatta 481 aagagggtta taatgaacga gaaaaatata aaacacagtc ACCATGAGTG ATAACACTGC 541 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA 601 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 661 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT 721 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 781 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA 841 ATCTGGAGCC GGTGAGCGTG GTTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA 901 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA 961 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAAgatt aataatctgg 1021 ctttttatat tctctctcta gtatataaac gcagaaaggc ccacccgaag gtgaccagtg 1081 tgaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct 1141 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga 1201 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat 1261 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt 1321 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg 1381 gatctttaaa tggagtgtcc tcttcccagt tttcgcaatc cacatcggcc agatcgttat 1441 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg 1501 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag 1561 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca 1621 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt 1681 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc 1741 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag 1801 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg 1861 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat 1921 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa 1981 ccttaaatac cagaaacgcg cccagctgtc tagggcggcg gCGCTaacgt cgtgactggg 2041 aaaaccctgg cgttacccaa cttaatcgcc atatgggaaa aatgccttta ttaccggaac 2101 ctatggtaaa aaaagcgatt ttaatgagct gatttcggta tacagttgag acaagatctt 2161 attattcaca ctttaaagga ggtttcctat gaaactgtta gcgattacat cttgtccgaa 2221 tggaatcgcc catacgtata tggcagcaga aaatctgcaa aaagctgctg acagactggg 2281 cgttagcatc aaagttgaga cccagggagg gattggtgtt gaaaataagc taaccgaaga 2341 ggaaattcgc gaagcggacg caatcattat tgctgcagac cgttcggtaa acaaagaccg 2401 gtttataggc aaaaaactgc tgtctgtggg ggtacaggac ggtatccgca aaccggaaga 2461 attgattcaa aaagctctta acggggatat accggtctat cgttccgcta caaaatcgga 2521 atccggtaat catcaggaga agaaacaaaa tccaatttac cgtcatttaa tgaacggcgt 2581 ttcatttatg gttcctttca ttgttgtcgg cggattattg atagcggttg cattaacgct 2641 tggaggggag aaaacaccta aaggcttagt tattccggat gattccttct ggaaaacgat 2701 tgaacagatc ggcagtgctt cattttcatt tatgattccg attttagccg gctatatcgc 2761 ctatagcatc gctgataaac cggggcttgt gccaggtatg atcggcggtt atattgcggc 2821 gactggaagt ttttatgaca gcgcaagcgg cgccggattc ctcgggggga ttattgccgg 2881 gtttttggcc ggatacgcgg cactttggat caaaaaatta aaagttccaa aagccatcca 2941 gccgattatg ccgattatta ttattccggt gtttgcatca ctgattgtag gtttagcgtt 3001 tgtgtttttg atcggcgcgc ctgtagctca aattttcgcc tctttaacag tttggctggc 3061 ggggatgaaa gggtcgagct cgatccttct ggcattgatt ttaggggcga tgatctcatt 3121 tgatatgggc ggtcccgtaa ataaggtggc gtttctgttt ggatcggcaa tgattggaga 3181 aggaaattat gaaattatgg gaccaattgc tgtcgccatt tgtatccccc cgattggcct 3241 tggcattgcg acgtttttag gaaaaagaaa attcgaggca tctcaaagag aaatggggaa 3301 agcggcattt acgatggggc tcttcggcat tactgaagga gcgattccat ttgcggcgca 3361 ggaccctctg cgcgttattc caagtattat ggcaggctca atgacaggtt ctgtgatagc 3421 catgattggc aatgtgggag acagagtggc gcacggcgga cctatcgttg cagtgcttgg 3481 cgcagttgat catgtcttga tgttttttat tgctgtgatt gcaggatctc ttgtcactgc 3541 tctgttcgtc aatgtactaa aaaaggatat tacggcgtct cctgtgctca gtgaaactgc 3601 accgacctcc gcgccaagtg aagctgcggc agcaaatgaa ataaagcagc caattcaaag 3661 ccaaaaagct gagatgtcgg aatttaaaaa actgacagac attatcagcc cggaattaat 3721 tgaaccaaat ttatcggggg aaacgagtga tgacatcata gatgaattga ttcagaaatt 3781 gtcacgaaga ggtgcgctgc tttcagagag cgggtttaaa caagccattt tgaatcgtga 3841 acaacaggga acaacggcga ttggcatgaa tatcgcgatt ccgcacggaa agtctgaggc 3901 ggtcagggag ccgagtgttg cctttgggat caaacgatca ggcgttgatt ggaatagcct 3961 ggatggatca gaagctaaat taatctttat gattgctgta ccgaaagaga gcgggggaaa 4021 ccagcatctc aaaatactgc agatgctatc tcgaaagcta atggatgaca attataggga 4081 gcggctgctc tccgtgcaaa caacagaaga agcatacaag ctgctagaag aaatcgaata 4141 aagcatatgg cgccataccg gtgatccCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4201 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4261 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4321 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4381 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4441 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4501 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4561 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 4621 GTAAGAGAAT TATGCAGTGC TGCCATAata ccttataaca taagtacgga tataatacgc 4681 aaaattgttt ttgatagtat agctaatgag atttatttaa tcgtggaata cgggtttgct 4741 aaaagattat taaatacaaa acgctcattg gcattacttt taatggcaga agttgatatt 4801 tctatattaa gtatggttcc aagagaatat tttcatccta aacctaaagt gaatagctca 4861 cttatcagat taagtagaaa aaaatcaaga atatcacaca aagataaaca aaagtataat 4921 tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa tatttacaaa aaatcaattt 4981 aacaattcct taaaacatgc aggaattgac gatttaaaca atattagctt tgaacaattc 5041 ttatctcttt tcaatagcta taaattattt aataagtaag ttaagggatg cataaactgc 5101 atcccttaac ttgtttttcg tgtgcctatt ttttgtgaat cgattatGGA G //