LOCUS construct_2 5151 bp DNA linear 30-JAN-2022 FEATURES Location/Qualifiers CDS 1536..2021 /label="AmpR" /color="#ccffcc" misc_feature 521..1535 /label="kanR" /color="#00ff00" CDS 602..1396 /label="aph3'" /color="#ccffcc" gene 602..1396 /label="aph3'" /color="#a6acb3" misc_feature 5..520 /label="flank_A" /color="#a6acb3" CDS 493..520 /label="erm" /color="#993366" gene 493..520 /label="erm" /color="#a6acb3" promoter 4168..4272 /label="AmpR promoter" /color="#ffffff" CDS 4342..4647 /label="AmpR" /color="#ccffcc" misc_feature 2026..4167 /label="manP" /color="#ff0000" CDS 2189..4141 /label="manP" /color="#993366" gene 2189..4141 /label="manP" /color="#a6acb3" misc_feature 4648..5147 /label="flank_B" /color="#a6acb3" CDS 4648..5079 /label="erm" /color="#993366" gene 4648..5079 /label="erm" /color="#a6acb3" ORIGIN 1 GGAGtacgcg ttaacccggg cccgcggatg catatgatca gatcctttaa ctctggcaac 61 cctcaaaatt gaatgagaca tgctacacct ccggataata aatatatata aacgtatata 121 gatttcataa agtctaacac actagactta tttacttcgt aattaagtcg ttaaaccgtg 181 tgctctacga ccaaaactat aaaaccttta agaactttct ttttttacaa gaaaaaagaa 241 attagataaa tctctcatat cttttattca ataatcgcat ccgattgcag tataaattta 301 acgatcactc atcatgttca tatttatcag agctcgtgct ataattatac taattttata 361 aggaggaaaa aatatgggca tttttagtat ttttgtaatc agcacagttc attatcaacc 421 aaacaaaaaa taagtggtta taatgaatcg ttaataagca aaattcatat aaccaaatta 481 aagagggtta taatgaacga gaaaaatata aaacacagtc gattaataat ctggcttttt 541 atattctctc tctagtatat aaacgcagaa aggcccaccc gaaggtgacc agtgtgaggt 601 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc 661 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc 721 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa 781 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa 841 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt 901 taaatggagt gtcctcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta 961 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt 1021 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt 1081 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc 1141 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc 1201 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg 1261 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca 1321 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata 1381 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca 1441 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa 1501 ataccagaaa cgcgcccagc tgtctagggc ggcggACCAT GAGTGATAAC ACTGCGGCCA 1561 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 1621 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 1681 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 1741 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 1801 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 1861 GAGCCGGTGA GCGTGGTTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 1921 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 1981 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACGCTaacgt cgtgactggg 2041 aaaaccctgg cgttacccaa cttaatcgcc atatgggaaa aatgccttta ttaccggaac 2101 ctatggtaaa aaaagcgatt ttaatgagct gatttcggta tacagttgag acaagatctt 2161 attattcaca ctttaaagga ggtttcctat gaaactgtta gcgattacat cttgtccgaa 2221 tggaatcgcc catacgtata tggcagcaga aaatctgcaa aaagctgctg acagactggg 2281 cgttagcatc aaagttgaga cccagggagg gattggtgtt gaaaataagc taaccgaaga 2341 ggaaattcgc gaagcggacg caatcattat tgctgcagac cgttcggtaa acaaagaccg 2401 gtttataggc aaaaaactgc tgtctgtggg ggtacaggac ggtatccgca aaccggaaga 2461 attgattcaa aaagctctta acggggatat accggtctat cgttccgcta caaaatcgga 2521 atccggtaat catcaggaga agaaacaaaa tccaatttac cgtcatttaa tgaacggcgt 2581 ttcatttatg gttcctttca ttgttgtcgg cggattattg atagcggttg cattaacgct 2641 tggaggggag aaaacaccta aaggcttagt tattccggat gattccttct ggaaaacgat 2701 tgaacagatc ggcagtgctt cattttcatt tatgattccg attttagccg gctatatcgc 2761 ctatagcatc gctgataaac cggggcttgt gccaggtatg atcggcggtt atattgcggc 2821 gactggaagt ttttatgaca gcgcaagcgg cgccggattc ctcgggggga ttattgccgg 2881 gtttttggcc ggatacgcgg cactttggat caaaaaatta aaagttccaa aagccatcca 2941 gccgattatg ccgattatta ttattccggt gtttgcatca ctgattgtag gtttagcgtt 3001 tgtgtttttg atcggcgcgc ctgtagctca aattttcgcc tctttaacag tttggctggc 3061 ggggatgaaa gggtcgagct cgatccttct ggcattgatt ttaggggcga tgatctcatt 3121 tgatatgggc ggtcccgtaa ataaggtggc gtttctgttt ggatcggcaa tgattggaga 3181 aggaaattat gaaattatgg gaccaattgc tgtcgccatt tgtatccccc cgattggcct 3241 tggcattgcg acgtttttag gaaaaagaaa attcgaggca tctcaaagag aaatggggaa 3301 agcggcattt acgatggggc tcttcggcat tactgaagga gcgattccat ttgcggcgca 3361 ggaccctctg cgcgttattc caagtattat ggcaggctca atgacaggtt ctgtgatagc 3421 catgattggc aatgtgggag acagagtggc gcacggcgga cctatcgttg cagtgcttgg 3481 cgcagttgat catgtcttga tgttttttat tgctgtgatt gcaggatctc ttgtcactgc 3541 tctgttcgtc aatgtactaa aaaaggatat tacggcgtct cctgtgctca gtgaaactgc 3601 accgacctcc gcgccaagtg aagctgcggc agcaaatgaa ataaagcagc caattcaaag 3661 ccaaaaagct gagatgtcgg aatttaaaaa actgacagac attatcagcc cggaattaat 3721 tgaaccaaat ttatcggggg aaacgagtga tgacatcata gatgaattga ttcagaaatt 3781 gtcacgaaga ggtgcgctgc tttcagagag cgggtttaaa caagccattt tgaatcgtga 3841 acaacaggga acaacggcga ttggcatgaa tatcgcgatt ccgcacggaa agtctgaggc 3901 ggtcagggag ccgagtgttg cctttgggat caaacgatca ggcgttgatt ggaatagcct 3961 ggatggatca gaagctaaat taatctttat gattgctgta ccgaaagaga gcgggggaaa 4021 ccagcatctc aaaatactgc agatgctatc tcgaaagcta atggatgaca attataggga 4081 gcggctgctc tccgtgcaaa caacagaaga agcatacaag ctgctagaag aaatcgaata 4141 aagcatatgg cgccataccg gtgatccCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 4201 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 4261 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 4321 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 4381 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 4441 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 4501 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 4561 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 4621 GTAAGAGAAT TATGCAGTGC TGCCATAata ccttataaca taagtacgga tataatacgc 4681 aaaattgttt ttgatagtat agctaatgag atttatttaa tcgtggaata cgggtttgct 4741 aaaagattat taaatacaaa acgctcattg gcattacttt taatggcaga agttgatatt 4801 tctatattaa gtatggttcc aagagaatat tttcatccta aacctaaagt gaatagctca 4861 cttatcagat taagtagaaa aaaatcaaga atatcacaca aagataaaca aaagtataat 4921 tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa tatttacaaa aaatcaattt 4981 aacaattcct taaaacatgc aggaattgac gatttaaaca atattagctt tgaacaattc 5041 ttatctcttt tcaatagcta taaattattt aataagtaag ttaagggatg cataaactgc 5101 atcccttaac ttgtttttcg tgtgcctatt ttttgtgaat cgattatGGA G //