LOCUS pDG1730 7602 bp DNA circular 30-JAN-2022 DEFINITION Cloning vector pDG1730 for ectopic integration into the Bacillus subtilis chromosome, complete sequence. FEATURES Location/Qualifiers source 1..7602 /label="source" /color="#ffffff" gene 115..2868 /label="amyE" /color="#a6acb3" CDS 115..635 /label="amyE" /color="#993366" gene 961..1713 /label="spc" /color="#a6acb3" CDS 961..1713 /label="spc" /color="#993366" CDS 1840..2868 /label="amyE" /color="#993366" gene 4231..4965 /label="erm" /color="#a6acb3" CDS 4231..4965 /label="erm" /color="#993366" gene 6532..7392 /label="bla" /color="#a6acb3" CDS 6532..7392 /label="bla" /color="#993366" rep_origin 5773..6361 /label="ori" /color="#ffff00" promoter 7393..7497 /label="AmpR promoter" /color="#ffffff" misc_feature 4534..5033 /label="flank_B" /color="#a6acb3" misc_feature 3743..4383 /label="flank_A" /color="#a6acb3" ORIGIN 1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga 61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt 121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat 181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt 241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg 301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg 361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg 421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa 481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat 541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca 601 catggaaaca cacaaattaa aaactggtct gatcggatcc tagaagctta tcgaattcct 661 gcagccctgg cgaatggcga ttttcgttcg tgaatacatg ttataataac tataactaat 721 aacgtaacgt gactggcaag agatattttt aaaacaatga ataggtttac acttacttta 781 gttttatgga aatgaaagat catatcatat ataatctaga ataaaattaa ctaaaataat 841 tattatctag ataaaaaatt tagaagccaa tgaaatctat aaataaacta aattaagttt 901 atttaattaa caactatgga tataaaatag gtactaatca aaatagtgag gaggatatat 961 ttgaatacat acgaacaaat taataaagtg aaaaaaatac ttcggaaaca tttaaaaaat 1021 aaccttattg gtacttacat gtttggatca ggagttgaga gtggactaaa accaaatagt 1081 gatcttgact ttttagtcgt cgtatctgaa ccattgacag atcaaagtaa agaaatactt 1141 atacaaaaaa ttagacctat ttcaaaaaaa ataggagata aaagcaactt acgatatatt 1201 gaattaacaa ttattattca gcaagaaatg gtaccgtgga atcatcctcc caaacaagaa 1261 tttatttatg gagaatggtt acaagagctt tatgaacaag gatacattcc tcagaaggaa 1321 ttaaattcag atttaaccat aatgctttac caagcaaaac gaaaaaataa aagaatatac 1381 ggaaattatg acttagagga attactacct gatattccat tttctgatgt gagaagagcc 1441 attatggatt cgtcagagga attaatagat aattatcagg atgatgaaac caactctata 1501 ttaactttat gccgtatgat tttaactatg gacacgggta aaatcatacc aaaagatatt 1561 gcgggaaatg cagtggctga atcttctcca ttagaacata gggagagaat tttgttagca 1621 gttcgtagtt atcttggaga gaatattgaa tggactaatg aaaatgtaaa tttaactata 1681 aactatttaa ataacagatt aaaaaaatta taaaaaaatt gaaaaaatgg tggaaacact 1741 tttttcaatt tttttgtttt attatttaat atttgggaaa tattcattct aattggtaat 1801 cagattttag aaaacaataa acccttgcat agggggatct cgacatggat gagcgatgat 1861 gatatccgtt taggctgggc ggtgatagct tctcgttcag gcagtacgcc tcttttcttt 1921 tccagacctg agggaggcgg aaatggtgtg aggttcccgg ggaaaagcca aataggcgat 1981 cgcgggagtg ctttatttga agatcaggct atcactgcgg tcaatagatt tcacaatgtg 2041 atggctggac agcctgagga actctcgaac ccgaatggaa acaaccagat atttatgaat 2101 cagcgcggct cacatggcgt tgtgctggca aatgcaggtt catcctctgt ctctatcaat 2161 acggcaacaa aattgcctga tggcaggtat gacaataaag ctggagcggg ttcatttcaa 2221 gtgaacgatg gtaaactgac aggcacgatc aatgccaggt ctgtagctgt gctttatcct 2281 gatgatattg caaaagcgcc tcatgttttc cttgagaatt acaaaacagg tgtaacacat 2341 tctttcaatg atcaactgac gattaccttg cgtgcagatg cgaatacaac aaaagccgtt 2401 tatcaaatca ataatggacc agacgacagg cgtttaagga tggagatcaa ttcacaatcg 2461 gaaaaggaga tccaatttgg caaaacatac accatcatgt taaaaggaac gaacagtgat 2521 ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc ggccaaaacc 2581 atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta taaacatgat 2641 gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac taaaaatgca 2701 gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc aaaagtgatt 2761 tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga ttacgtgcta 2821 aatggtttat ataatgactc gggcttaagc ggttctcttc cccattgagg gcaaggctag 2881 acgggactta ccgaaagaaa ccatcaatga tggtttcttt tttgttcata aatcagacaa 2941 aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata taaatgtgaa atacttcaca 3001 aacaaaaaga catcaaagag aaacataccc tgcaaggatg ctgatattgt ctgcatttgc 3061 gccggagcaa accaaaaacc tggtgagaca cgccttgaat tagtagaaaa gaacttgaag 3121 attttcaaag gcatcgttag tgaagtcatg gcgagcggat ttgacggcat tttcttagtc 3181 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg 3241 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt 3301 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg 3361 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc 3421 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctactga 3481 agtggatttc tttaagagct cctttaactt cctcaccagt agttgtatcg gtaccataag 3541 tagaagcagc aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct 3601 tactggacat cggcagttct tcgaacagtg cgccaactac cagctctttc tgcagttcat 3661 tcagggcacc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca 3721 tcctgtctct tgatccatgg attacgcgtt aacccgggcc cgcggatgca tatgatcaga 3781 tcctttaact ctggcaaccc tcaaaattga atgagacatg ctacacctcc ggataataaa 3841 tatatataaa cgtatataga tttcataaag tctaacacac tagacttatt tacttcgtaa 3901 ttaagtcgtt aaaccgtgtg ctctacgacc aaaactataa aacctttaag aactttcttt 3961 ttttacaaga aaaaagaaat tagataaatc tctcatatct tttattcaat aatcgcatcc 4021 gattgcagta taaatttaac gatcactcat catgttcata tttatcagag ctcgtgctat 4081 aattatacta attttataag gaggaaaaaa tatgggcatt tttagtattt ttgtaatcag 4141 cacagttcat tatcaaccaa acaaaaaata agtggttata atgaatcgtt aataagcaaa 4201 attcatataa ccaaattaaa gagggttata atgaacgaga aaaatataaa acacagtcaa 4261 aactttatta cttcaaaaca taatatagat aaaataatga caaatataag attaaatgaa 4321 catgataata tctttgaaat cggctcagga aaaggccatt ttacccttga attagtaaag 4381 aggtgtaatt tcgtaactgc cattgaaata gaccataaat tatgcaaaac tacagaaaat 4441 aaacttgttg atcacgataa tttccaagtt ttaaacaagg atatattgca gtttaaattt 4501 cctaaaaacc aatcctataa aatatatggt aatatacctt ataacataag tacggatata 4561 atacgcaaaa ttgtttttga tagtatagct aatgagattt atttaatcgt ggaatacggg 4621 tttgctaaaa gattattaaa tacaaaacgc tcattggcat tacttttaat ggcagaagtt 4681 gatatttcta tattaagtat ggttccaaga gaatattttc atcctaaacc taaagtgaat 4741 agctcactta tcagattaag tagaaaaaaa tcaagaatat cacacaaaga taaacaaaag 4801 tataattatt tcgttatgaa atgggttaac aaagaataca agaaaatatt tacaaaaaat 4861 caatttaaca attccttaaa acatgcagga attgacgatt taaacaatat tagctttgaa 4921 caattcttat ctcttttcaa tagctataaa ttatttaata agtaagttaa gggatgcata 4981 aactgcatcc cttaacttgt ttttcgtgtg cctatttttt gtgaatcgat tatgtctttt 5041 gcgcagtcgg cttaaaccag ttttcgctgg tgcgaaaaaa gagtgtcttg tgacacctaa 5101 attcaaaatc tatcggtcag atttataccg atttgatttt atatattctt gaataacata 5161 cgccgagtta tcacataaaa gcgggaacca atcatcaaat ttaaacttca ttgcataatc 5221 cattaaactc ttaaattcta cgattccttg ttcatcaata aactcaatca tttctttaat 5281 taatttatat ctatctgttg ttgttttctt taataattca tcaacatcta caccgccata 5341 aactatcata tcttcttttt gatatttaaa tttattagga tcttaaggcc taggtctaga 5401 gtctttgttt tgacgccatt agcgtacgta acaatcctcg ttaaaggaca aggacctgag 5461 cggaagtgta tcgtacagta gacggagtat actagtatag tctatagtcc gtggaattat 5521 tatatttatc tccgacgata ttctcatcag tgaaatccag ctggagttct ttagcaaatt 5581 tttttattag ctgaacttag tattagtggc catactcctc caatccaaag ctatttagaa 5641 agattactat atcctcaaac aggcggtaac cggcctcttc atcgggaatg cgcgcgacct 5701 tcagcatcgc cggcatgtcc ccctggcgga cgggaagtat ccagctcgag gtcgggccgc 5761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 6181 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 6241 gaagccagtt accttcggaa aaagagttga tagctcttga tccggcaaac aaaccaccgc 6301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 6481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 6541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 6601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 6661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 6721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 6781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 6841 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 6901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 6961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 7141 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 7201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 7261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 7321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 7441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 7501 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 7561 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa tt //