Strains

Plants

Leucaena leucocephala

Commonly known as white leadtree (also known in Hawaii as Koa Haole), this is one of the fastest growing trees there is, being able to go seed-to-seed in less than a year, while also being a legume (fixing its own nitrogen!)

Papers

General facts

Yeast

Kluyveromyces lactis pGKL1/2

Kluyveromyces lactis NRRL Y-1140 has the pGKL1 and pGKL2 plasmids.

Kluyveromyces lactis NRRL Y-1140

Papers

Origin of Lactose Fermentation in Kluyveromyces lactis by Interspecies Transfer of a Neo-functionalized Gene Cluster during Domestication

An efficient method to optimize Kluyveromyces lactis gene targeting

Medium

NRRL Medium 6

YPD also works

Komagataella phaffii (Pichia pastoris)

Pichia pastoris has an interesting phylogenetic tree. Basically all strains that are commonly used are derived from CBS7435, but this is a patented strain with restrictions. A genomic analysis of NRRL Y-7556, which was found on an oak tree, shows only 3-4 nucleotide mutations from the CBS7435 strain, and is available in the public NRRL repository. Therefore, we're going to use NRRL Y-7556 for all experiments.

Papers

Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris)

Development of a general defined medium for Pichia pastoris

Medium

NRRL Medium 5

RDM defined medium

Saccharomyces cerevisiae

I wanted BY4741 and BY4742, since these are the two haploid strains used in the Yeast Deletion Project. The only place I could find them perhaps without detrimental terms and conditions was Euroscarf, but unfortunately their MTA directed me to a test PDF. We will see if we can get these strains under good terms and conditions.

Yeast Deletion Project

Euroscarf

The test PDF

Kluyveromyces marxianus

The target of the genome minimization efforts for eukaryotes. They used K. marxianus NRRL Y-6860, and so we will use this as well. In particular, K. marxianus is interesting because it grows so fast - colonies are pickable after an overnight, unlike S. cerevisiae.

Genome minimization using K. marxianus (timestamp 9:48). Note this project has been discontinued.

K. marxianus NRRL Y-6860

Papers

Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host

Gross Chromosomal Rearrangements in Kluyveromyces marxianus Revealed by Illumina and Oxford Nanopore Sequencing

Medium

NRRL Medium 6

YPD also works

Bacteria

Mesoplasma florum

Unfortunately, all current resources for Mesoplasma florum L1 (ATCC 33453 / NBRC 100688 / NCTC 11704) are all incompatible with the OpenMTA. I am asked S├ębastien Rodrigue and Tom Knight if they possibly had any strains available.