Commonly known as white leadtree (also known in Hawaii as Koa Haole), this is one of the fastest growing trees there is, being able to go seed-to-seed in less than a year, while also being a legume (fixing its own nitrogen!)
Kluyveromyces lactis NRRL Y-1140 has the pGKL1 and pGKL2 plasmids.
Kluyveromyces lactis NRRL Y-1140
An efficient method to optimize Kluyveromyces lactis gene targeting
Pichia pastoris has an interesting phylogenetic tree. Basically all strains that are commonly used are derived from CBS7435, but this is a patented strain with restrictions. A genomic analysis of NRRL Y-7556, which was found on an oak tree, shows only 3-4 nucleotide mutations from the CBS7435 strain, and is available in the public NRRL repository. Therefore, we're going to use NRRL Y-7556 for all experiments.
Development of a general defined medium for Pichia pastoris
I wanted BY4741 and BY4742, since these are the two haploid strains used in the Yeast Deletion Project. The only place I could find them perhaps without detrimental terms and conditions was Euroscarf, but unfortunately their MTA directed me to a test PDF. We will see if we can get these strains under good terms and conditions.
The target of the genome minimization efforts for eukaryotes. They used K. marxianus NRRL Y-6860, and so we will use this as well. In particular, K. marxianus is interesting because it grows so fast - colonies are pickable after an overnight, unlike S. cerevisiae.
Genome minimization using K. marxianus (timestamp 9:48). Note this project has been discontinued.
Engineering Kluyveromyces marxianus as a Robust Synthetic Biology Platform Host
Unfortunately, all current resources for Mesoplasma florum L1 (ATCC 33453 / NBRC 100688 / NCTC 11704) are all incompatible with the OpenMTA. I am asked Sébastien Rodrigue and Tom Knight if they possibly had any strains available.